Genome Reduction

WP1 - Reduction and engineering of the B. subtilis genome is dedicated to the construction of B. subtilis strains modified à la carte. WP1 will generate strains with reduced genome, build synthetic pathways into selected B. subtilis cells, fuse fluorescent reporters to genes of interest to monitor their expression and fine tune the operation of synthetic modules, and create mutations altering the coupling of growth rate with metabolic activity. WP1 will also develop novel chromosome engineering tools to improve the efficiency of chromosome modification and make the technique amenable to high throughput.